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Anti-GNAT1 Antibody, clone 11A8.1
产品别名
Anti-GNAT1 Antibody, clone 11A8.1
Guanine nucleotide-binding protein G(t) subunit alpha-1, Transducin alpha-1 chain
基本信息
eCl@ss | 32160702 |
General description【一般描述】 | Guanine nucleotide-binding protein G(t) subunit alpha-1 (UniProt P11488; also known as Transducin alpha-1 chain) is encoded by the GNAT1 (also known as GNATR, CSNB1G, CSNBAD3) gene (Gene ID 2779) in human. Heterotrimeric G proteins are activated by G protein-coupled receptors (GPCRs) and are composed of alpha (α), beta (β), and gamma (γ) subunits. There exists also monomeric Ras superfamily of small GTPases that are commonly referred to as small G-proteins. G-proteins mediate signal transduction initiated by GPCRs. Ligand binding activates GPCRs by inducing a conformational change. The activated GPCRs catalyze the GDP-to-GTP exchange of the Gα subunit, causing a dissociation of the Gβγ dimer from the now GTP-bound Gα subunit. The Gα subunit gradually hydrolyzes the bound GTP to GDP and eventually re-associates with Gβγ and re-starts the cycle. There exist at least 20 different Gα subunits, which are separated into four main groups, Gi, Gs, Gq, and G12/13. Eight different genes code for Gi alpha subunits, including GNAT1 and GNAT2 that code for Gt in the rod (rTα by GNAT1) and cone (cTα by GNAT2) cells of the retina, where they function as the alpha subunit of the trimeric G protein transducin. The α-subunit binds GTP and activates cyclic GMP phosphodiesterase (PDE), whereas the β and γ subunits mediate interaction with rhodopsin. Mutations in RHO, PDE6B, and GNAT1 genes are associated with autosomal dominant congenital stationary night blindness (CSNB), and patients with mutations in these genes exhibit the Riggs- or Schubert-Bornschein-type electronegative electroretinogram (ERG). |
Specificity【特异性】 | Clone 11A8.1 was raised against human Gt alpha-1 subunit (GNAT1) recombinant fragment. Cross-reactivity toward human Gt alpha-2 subunit (GNAT2) has not been determined. |
Immunogen【免疫原】 | GST-tagged recombinant human GNAT1 internal fragment. |
Application【应用】 | Anti-GNAT1 Antibody, clone 11A8.1 is an antibody against GNAT1 for use in Western Blotting. |
Quality【质量】 | Evaluated by Western Blotting in human retina tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected GNAT1 in 10 µg of human retina tissue lysate. |
Physical form【外形】 | Protein G purified. Format: Purified |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | 11A8.1, monoclonal |
species reactivity | human |
technique(s) | western blot: suitable |
isotype【同位素/亚型】 | IgG2aκ |
NCBI accession no.【NCBI登记号】 | NP_000163 |
UniProt accession no.【UniProt登记号】 | P11488 |
产品说明
Target description【目标描述】 | ~40 kDa observed. 39.91 kDa calculated. |
【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |