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肠激酶 来源于猪肠

Enterokinase from porcine intestine
产品编号:3081081
规格:≥0.5 units/mg solid
CAS NO:9014-74-8
包装规格:1500 UNITS
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品编号包装单位单价(元)国内现货国外库存询价单
30810811500 UNITS4640
产品别名

9014-74-8

肠激酶 来源于猪肠

Enterokinase from porcine intestine

Enteropeptidase

肠肽酶

基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】
3.4.21.9 ( BRENDA | IUBMB )
EC number【EC 号】
232-761-1
MDL number
MFCD00131020
eCl@ss
32160410
NACRES
NA.54
Application【应用】
The enzyme from Sigma has been used for the activation of trypsinogen in order to measure the activity of trypsin in hog pancreas. The study showed that antimicrobial treatment reduces intestinal microflora and improves protein digestive capacity without changes in villous structure of weanling pigs.
Enterokinase from porcine intestine has been used in a study to report a new experimental model of the anomalous pancreatico-biliary junction. Enterokinase from porcine intestine has also been used in a study to investigate the insulinotropic region of the gastric inhibitory polypeptide.
Biochem/physiol Actions【生化/生理作用】
Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.
Unit Definition【单位定义】
One unit will produce 1.0 nanomole of trypsin from trypsinogen per min at pH 5.6 at 25 ℃.
产品性质
Quality Level【质量水平】
200
form【形式】
salt-free, lyophilized powder
Specific Activity
≥0.5 units/mg solid
mol wt【分子量】
150 kDa
storage temp.【储存温度】
−20℃
packaging【包装】
1500 units in poly bottle
安全信息
Storage Class Code【储存分类代码】
11 - Combustible Solids
WGK
WGK 3
Personal Protective Equipment【个人防护装备】
Eyeshields, Gloves, type N95 (US)
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